Based on cross-fault observation data, large area leveling data and seismic activity data of the periphery of epicenter area and Bayanhar block around the Jiuzhaigou M_S7.0 earthquake, and 3D fault activity parameters and leveling adjustment calculation method, we analysed comprehensively regional crustal deformation before and after earthquake and the relationship between the Bayanhar block integrity movement and earthquake. The conclusions are as follow:① Before the Jiuzhaigou earthquake, there were abnormal changes in varying degrees in the west Qinling Mountain fault zone, the Liupan Mountain Fault zone, the Longmen Mountain Fault zone and the Xianshuihe fault zone, and the deformation in the epicentral region was not significant. ② Through the analysis of the fault activity and seismicity anomalies around the Bayanhar block, the block SE clockwise accelerated motion caused the stress accumulation of epicentral region, and then the earthquake occurred. At the same time, several strong earthquakes including Lushan earthquake has a certain accelerating effect on the Jiuzhaigou earthquake;③After the Jiuzhaigou earthquake, the regional stress accumulation has been released to some extent, and the stress may accelerate accumulation in the "three road" area.