The data used in this study are continuous seismic waveforms recorded at broadband seismic stations including the 387 stations of China National and Regional Seismic Network in 2009 and 2010. Cross-correlations are computed in daily segment to retrieve the reliably empirical Rayleigh wave Green's function. Rayleigh wave phase velocity dispersion curves are measured using a phase-matched filter and frequency time analysis technique. We apply seismic ambient noise tomography to image the different period phase velocity maps of South China. Our results indicate that the phase velocity of the whole lithosphere in South China is relatively uniform,which also reflects that South China as a whole is characterized of the relative stability. Our results agree that there have been no intense tectonic activities in South China since late Mesozoic. Although the velocity structure of the whole lithosphere in South China is relatively uniform,distinct velocity differences appear in the west of the Yangtze Block,Sichuan Basin,the east of Yangtze Block and Cathaysia Block,namely,the 8-10s period phase velocity of Cathaysia Block is higher than the west of the Yangtze Block,Sichuan-Yunnan region and Sichuan Basin. The Sichuan Basin has the lowest velocity because of the relatively thick sedimentary layer. The velocity of the Cathaysia Block in the 10-30s period phase is higher than those of the Yangtze Block and Sichuan Basin and the Sichuan-Yunnan region has the lowest velocity. In the 35s period phase,the velocities of the Yangtze Block and Cathaysia Block and Sichuan Basin are basically the same and higher than that of the Sichuan-Yunnan region. This is consistent with the feature that the crustal thickness in South China is obviously smaller than that of the Sichuan-Yunnan region.