In this paper, the characteristics of the co-seismic response of the well water level from 2004 to 2016 and the relationship between the tidal parameters before and after the earthquake are analyzed. The results show that under the premise that the main flow direction of the Mile well is the vertical flow, natural(earthquake) and artificial(washing, plant modification) factors can change the direction of the aquifer flow. When the tidal factor and the phase difference change in the opposite direction before the earthquake, the earthquake with larger energy can change the direction of the water flow, so that the tidal factor and the phase difference change in the same direction after the earthquake, and the direction of the water flow changes from vertical to radial dominance. The earthquake with less energy only changed the permeability coefficient of the aquifer, but does not change the direction of its water flow, and still dominates the vertical flow after the earthquake. When the tidal factor and phase difference before the earthquake change in the same direction, the earthquake with larger energy can increase the permeability coefficient of the radial aquifer, the direction of the water flow is unchanged, and the radial direction is still dominant. By analyzing the changes of tidal parameters, we can further know the change of the well-aquifer radial and vertical permeability coefficient, which lays a foundation for the accurate calculation of aquifer parameters.