In the study of underground fluids in earthquakes,groundwater recharge and circulation are one of the important research contents. Hydrogen and oxygen isotope tracing technology is one of the most commonly used methods in studying the process. By means of the hydrogen and oxygen isotope determination,we analyze the samples from the groundwater in Well No.2 of the Jiujiang Seismic Station,atmospheric precipitation,the water flowing from the surrounding Tianhuajing Reservoir and a kind of alpine spring water named Mawei Water. The results show that groundwater δ¹⁸O is between -7.59‰ and -6.09‰ and average value is -6.99‰ and that its δD is from -45.22‰ to -39.69‰ and average value-42.32‰. At the same time,the coefficient of variance is separately 0.09 and 0.16. As for the atmospheric precipitation,its atmospheric precipitation ranges from -13.00‰ to -1.27‰ and average value-4.74‰,compared with the average value-46.87‰,δD changes between 96.13‰ and -4.74‰ with 0.40 and 0.56 as coefficient of variance. Obviously,hydrogen and oxygen isotope of groundwater changes more stably than precipitation's. The hydrogen and oxygen isotope of atmospheric precipitation is reflected by obvious precipitation effect in the summer half year,while the temperature effect in the winter half year is not obvious. The hydrogen and oxygen isotope of groundwater has no significant effects on precipitation and temperature. Hydrogen and oxygen isotope and excess enthalpy reveal that groundwater experienced evaporation fractionation before infiltration replenishment and exchanged with surrounding rocks totally in ¹⁸O. The recharge elevations calculated by δ¹⁸O and δD are about 647m and 440m respectively. Generally speaking,the groundwater in the Jiujiang seismic station belongs to a type of atmospheric origin,and its circulation process is a pressure-bearing artesian well formed by the supplement of a relatively stable fissure water.