Load/unload response ratio(LURR)is a method based on the dynamic evolution characteristics of constitutive relationship of rock media for earthquake prediction. On June 26,2020,the earthquake with magnitude 6.4 occurred in Yutian,Xinjiang. We have observed the LURR anomaly based on Benioff strain as the response before the earthquake. The characteristics of the anomaly in temporal and spatial evolution show that the stress state of the source area has changed significantly in 2019. LURR anomalies show the intermediate-to-short-term earthquake potential,which markedly enhanced in 2019 and decreased in 2020. After the earthquake,we calculated the LURR anomaly time series curves of six items of long and short polar distances of Hetian electric field 280km away from the epicenter. The results show that the synchronous variation of LURR anomaly gradually increases in in both north-south direction and north-west direction of the long and short polar distance of Hetian electric field about 1~3 months before the earthquake,which may reflect the electrokinetic effect of fluid migration in the fracture due to the generation and expansion of micro fractures in the seismogenic area. Comprehensive analysis of the characteristics of LURR anomaly evolution with two different responses is helpful to understand the process of earthquake generation and evaluate the trend of seismic activity more accurately.